Plasmid

Part:BBa_I713017:Design

Designed by: Zhenyu Shi   Group: iGEM07_Tsinghua   (2007-10-25)


pLZHindIII2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2394
    Illegal XbaI site found at 3595
    Illegal PstI site found at 3589
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 673
    Illegal PstI site found at 3589
    Illegal NotI site found at 3601
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 688
    Illegal BamHI site found at 2656
    Illegal XhoI site found at 684
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2394
    Illegal XbaI site found at 3595
    Illegal PstI site found at 3589
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2394
    Illegal XbaI site found at 3595
    Illegal PstI site found at 3589
    Illegal NgoMIV site found at 132
    Illegal NgoMIV site found at 2409
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3335
    Illegal BsaI.rc site found at 4977
    Illegal SapI site found at 3894
    Illegal SapI.rc site found at 1547


Design Notes

For effectively producing HindIII digested linear DNA.


Source

pLZZGPp pGL3-BASIC

References